AGAP2 antibody (Internal Region)

Catalog No. ABIN452299

Product Details anti-AGAP2 Antibody

Target: AGAP2 (ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AGAP2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody reacts to CENTG1.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-QASLDSIREAVINSQ, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/8000. Western blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for AGAP2

Target: AGAP2 (ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2))

Alternative Name: AGAP2 / CENTG1 (AGAP2 Products)

Synonyms: zgc:153779 antibody, CENTG1 antibody, GGAP2 antibody, PIKE antibody, Centg1 antibody, mKIAA0167 antibody, Pike antibody, ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody, AGAP2 antibody, agap2 antibody, Agap2 antibody

Background: Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms, a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis. Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes. In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.Synonyms: Arf-GAP GTPase ANK repeat and PH domain-containing protein 2, Centaurin-gamma-1, GGAP2, GTP-binding and GTPase-activating protein 2, KIAA0167, PIKE, Phosphatidylinositol-3-kinase enhancer

Gene ID: 116986

NCBI Accession: NP_055585

UniProt: Q99490