GGA1
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
ELISA: 1/10,000 - 1/50,000. Western Blot: 1/500 - 1/3,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.35 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
GGA1 (also known as ADP-ribosylation factor binding protein, Golgi-localized, gamma earcontaining, ARF-binding protein 1, Gamma-adaptin related protein 1) plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. This protein mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. GGA1 is a monomeric protein that interacts with NECAP1, GGA2 and GGA3 as well as RABEP1, RABGEF1 and type-I membrane proteins SORT1, SORL1, LRP3, M6PR/CDMPR, IGF2R/CI-MPR and BACE1. GGA1 is a ubiquitously membrane-associated protein that is localized to TGN and endosomes. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.Synonyms: ADP-ribosylation factor-binding protein GGA1, Gamma-adaptin-related protein 1, Golgi-localized gamma ear-containing ARF-binding protein 1