PRNP antibody (Internal Region)

Catalog No. ABIN374705

Product Details anti-PRNP Antibody

Target: PRNP (Prion Protein (PRNP))

Binding Specificity: AA 143-153, Internal Region

Reactivity: Human, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This PRNP antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-SDYEDRYYRE N

Specificity: This antibody detects an internal region of Prion Protein.

Cross-Reactivity (Details): Species reactivity (expected):Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_000302.1. Genename: PRNP

Application Details

Application Notes: ELISA: 1/64000. Western Blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PRNP

Target: PRNP (Prion Protein (PRNP))

Alternative Name: CD230 / PrP (PRNP Products)

Synonyms: ASCR antibody, AltPrP antibody, CD230 antibody, CJD antibody, GSS antibody, KURU antibody, PRIP antibody, PrP antibody, PrP27-30 antibody, PrP33-35C antibody, PrPc antibody, p27-30 antibody, Prn antibody, Prn-p antibody, prp antibody, cjd antibody, prion antibody, prp(c) antibody, MGC84114 antibody, PRP antibody, PRPC antibody, Prp antibody, SIP antibody, PRNP antibody, AA960666 antibody, AI325101 antibody, PrP antibody, PrPC antibody, PrPSc antibody, Prn-i antibody, Sinc antibody, prP27-30 antibody, prP33-35C antibody, PrP-1 antibody, PrP1 antibody, prnprs1 antibody, stPrP-1 antibody, zgc:123004 antibody, prion protein antibody, prion protein S homeolog antibody, prion protein b antibody, PRNP antibody, Prnp antibody, prnp.S antibody, prnpb antibody

Background: Prion protein PrP is a membrane glycosylphosphatidylinositol anchored glycoprotein that tends to aggregate into rod like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt Jakob disease, fatal familial insomnia, Gerstmann Straussler disease, Huntington disease like 1, and kuru. Two transcript variants encoding the same protein have been found for this gene. All prion diseases share the same molecular pathogenic mechanism that involves conversion of normal cellular prion protein (PrPc) into a form that is insoluble in non ionic detergent and partially resistant to proteases (PrPsc). Both PrPsc and PrPc are encoded within a single exon of a chromosomal gene as a protein of ~ 250 amino acids. Many mammalian PrPs have a 22 amino acid N terminal signal sequence and 23 amino acid C terminal signal sequence encoding for attachment of a glycosylphosphatidylinositol anchor. The mature protein of 209 amino acids contains one disulfide bond and has two sites of asparagine linked glycosylation.Synonyms: ASCR, Major prion protein, PRIP, PRNP, PrP27-30, PrP33-35C

Gene ID: 5621, 9606

UniProt: P04156

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation