Fibronectin antibody

Catalog No. ABIN5564237

Product Details anti-Fibronectin Antibody

Target: Fibronectin

Reactivity: Human, Mouse, Rat, Pig, Cow, Chicken, Dog, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Functional Studies (Func)

Specificity: Recognizes an epitope located in the EDA sequence of human cellular fibronectin (FN).

Cross-Reactivity: Chicken, Cow (Bovine), Dog (Canine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Does not detect extracellular FN (plasma FN). Cross-reacts with mouse, rat, monkey, pig, dog, cow and chicken fibronectin EDA (according to literature). Cross-reacts with mouse, rat, monkey, pig, dog, cow and chicken fibronectin EDA (according to literature).

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.001EU/μg purified protein (LAL test, Lonza).

Immunogen: Recombinant Extra Domain A (EDA) region of human cellular fibronectin.

Clone: IST-9

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In 20 mM sodium phosphate buffer, pH 7.6 containing 150 mM sodium chloride.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details for Fibronectin

Target: Fibronectin

Abstract: Fibronectin Products

Synonyms: FN antibody, cig antibody, fibronectin antibody, finc antibody, lets antibody, msf antibody, CIG antibody, ED-B antibody, FINC antibody, FNZ antibody, GFND antibody, GFND2 antibody, LETS antibody, MSF antibody, E330027I09 antibody, Fn antibody, Fn-1 antibody, FIBNEC antibody, fn-1 antibody, fibronectin 1 antibody, FN1 antibody, fn1 antibody, Fn1 antibody

Background: Fibronectin is a major component of the extracellular matrix that binds to integrins, cell surfaces and various compounds including collagen, fibrin, heparin, DNA and actin. It occurs in two main forms: plasma and cellular fibronectin. EDA (Extra Domain A)-containing cellular fibronectin (EDA-FN) plays a major role in cell adhesion, growth, migration and differentiation and it is important for processes such as wound healing and embryonic development. It is associated with fibroblast differentiation by increasing alpha-SMA expression, collagen deposition, cell contractility and focal adhesion kinase (FAK) activation. EDA-FN promotes cell spread by interacting with the integrins alpha5-beta1, alpha4-beta1 and alpha9-beta1 and has been shown to promote angiogenesis, lymphangiogenesis and metastasis of colorectal tumors. In transformed or tumor-derived cells the EDA segment is about 10-times higher than in fibronectin from normal fibroblasts and it may therefore be a significant marker for malignancy.