Recombinant ADAM17 antibody (His tag)
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- Target See all ADAM17 Antibodies
- ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human
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Host
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Human
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Clonality
- Monoclonal
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Conjugate
- This ADAM17 antibody is conjugated to His tag
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Application
- Functional Studies (Func)
- Specificity
- Recognizes the catalytic and non-catalytic domain of human ADAM17 (TACE) through its variable light (VL) domain and variable heavy (VH) domain, respectively. Does not bind recombinant mouse ADAM17 ectodomain.
- Cross-Reactivity
- Human
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.001EU/μg
- Immunogen
- Recombinant human ADAM17 (TACE) ectodomain tagged to biotin.
- Clone
- D1(A12)
- Isotype
- Kappa
- Top Product
- Discover our top product ADAM17 Primary Antibody
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- Comment
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This cross-domain human antibody is a selective ADAM17 (TACE) antagonist and provides a unique alternative to small molecule metalloprotease inhibition. ADAM multidomain topology was exploited by first isolating an inhibitory human antibody (D1) that bound ADAM17-specific non-catalytic regions exclusively through its variable heavy (VH) domain. A D1-VH biased scFv phage-display library was then used to selectively isolate a new variable light (VL) chain that could simultaneously bind to the ADAM17 catalytic domain. This monovalent Fab Antibody has been shown by C.J. Tape, et al., PNAS 108, 5578 (2011) to be as active as the divalent antibody .
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- In PBS.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C. Stable for at least 3 months after receipt when stored at +4°C. - Expiry Date
- 12 months
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- Target
- ADAM17 (ADAM Metallopeptidase Domain 17 (ADAM17))
- Alternative Name
- ADAM17 (ADAM17 Products)
- Synonyms
- ADAM18 antibody, CD156B antibody, CSVP antibody, NISBD antibody, TACE antibody, CD156b antibody, Tace antibody, adam17 antibody, si:dkey-81b15.2 antibody, adam18 antibody, cd156b antibody, csvp antibody, tace antibody, fc20b01 antibody, wu:fc20b01 antibody, zgc:63886 antibody, ADAM metallopeptidase domain 17 antibody, a disintegrin and metallopeptidase domain 17 antibody, ADAM metallopeptidase domain 17b antibody, ADAM metallopeptidase domain 17 L homeolog antibody, ADAM metallopeptidase domain 17a antibody, ADAM17 antibody, Adam17 antibody, adam17b antibody, adam17.L antibody, adam17a antibody
- Background
- ADAM17 (Disintegrin and metalloproteinase domain-containing protein 17), also called TACE (Tumor necrosis factor-alpha-converting enzyme) is the prototype of the ADAM family of ectodomain shedding proteases (sheddase). ADAM17 is understood to be involved in the processing of TNF-alpha at the surface of the cell and from within the intracellular membranes of the trans-Golgi network. This process involves the cleavage and release of a soluble ectodomain from membrane-bound pro-proteins (such as pro-TNF-alpha) and is of known physiological importance. ADAM17 is responsible for the processing of a diverse variety of membrane-anchored cytokines, cell adhesion molecules, receptors, ligands and enzymes, including cleaving epidermal growth factor receptor (EGFR) ligands and extracellular Notch1. The proteolytic cleavage is an indispensable activation event for many of these substrates, ADAM17 has emerged as an attractive therapeutic target for the treatment of inflammatory diseases (e.g. rheumatoid arthritis) or inflammation associated cancer.
- UniProt
- P78536
- Pathways
- Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus
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