This APP antibody is conjugated to Alkaline Phosphatase (AP)
Application
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Dot Blot (DB)
Specificity
PAB28937 recognizes all types of amyloid oligomers, likely with a peptide backbone epitope that is common in amyloid oligomers but not in native proteins, amyloidogenic monomer or mature amyloid fibrils. A 1:1000 dilution of PAB28937 was sufficient for detection of amyloid oligomers in 10 μg of mouse brain lysates by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cross-Reactivity
Human, Mouse, Rat
Immunogen
Synthetic molecular mimic of soluble APP oligomers.
Dot blot ELISA Immunofluorescence Immunohistochemistry (1:1000-10000) Immunoprecipitation (1:1000) Western Blot The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS pH 7.4, 50 % glycerol and 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store at 4 °C. Aliquot to avoid repeated freezing and thawing.