Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Purpose
Rabbit polyclonal antibody raised against synthetic peptide of COL1A1.
Specificity
This antibody has been directly tested for reactivity in human, mouse and rat. Based on the homology of the antigen this antibody is expected to recognize the collagen I 1 polypeptide in most, if not all, species of mammals, birds, amphibians, and fishes. specific to the propeptide portion of the ~180 KDa collagen I alpha1 polypeptide in human lung fibroblast extract. This antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90°C in 10 mM sodium citrate (pH 6.0)/ 0.1% Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, This antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized.
Cross-Reactivity
Avian, Fish, Human, Mammalian, Mouse, Rat
Immunogen
A synthetic peptide corresponding to human COL1A1.