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His Tag antibody (N-Term)

Name: His Tag antibody (N-Term)
SKU: ABIN5579851
Price: 686.67 USD
Availability: InStock

WB, IP, FACS, ICC, IHC (p) Host: Rabbit Monoclonal RM146 unconjugated

Western blot analysis of 293T cell Lane 1: untransfected with His-Tag fusion protein X, Lane 2: transfected with His-Tag fusion protein X, Lane 3: E. (His Tag antibody (N-Term))

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of naive HepG2 cells (Negative control) (A) and 293T cells expressing His-Tag nuclear protein X (B) with His-Tag monoclonal antibody, clone RM146 . (His Tag antibody (N-Term))

IHC

Immunoprecipitation analysis of 293T cells expressing a His-Tag fusion protein with His-Tag monoclonal antibody, clone RM146 . (His Tag antibody (N-Term))

3 images

Catalog No. ABIN5579851

Datasheet as PDF

Target See all His Tag Antibodies

His Tag
Binding Specificity
16

2

1
N-Term

Reactivity

Please inquire
Host
93

55

12

9

8

7

2

1
Rabbit
Compatible Secondaries
Clonality
105

69

10
Monoclonal
Conjugate
90

22

14

12

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is un-conjugated
Application
152

103

37

27

25

16

14

14

13

13

13

7

7

6

5

5

4

4

3

3

2

2

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose

Recombinant rabbit monoclonal antibody raised against of His-Tag.

Specificity

This antibody reacts to recombinant proteins containing the 6X His-Tag or 10X His-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

Immunogen

Original antibody is raised against a mixture of synthetic peptides corresponding to N-terminus region of 6X His-Tag and C-terminus region of 6X His-Tag.

Clone

RM146

Isotype

IgG

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Application Notes

Flow Cytometry (0.5 μg/mL-2 μg/mL)
Immunocytochemistry (0.5 μg/mL-2 μg/mL)
Immunohistochemistry (0.1 μg/mL-1 μg/mL)
Immunoprecipitation (0.5 μg/mL-2 μg/mL)
Western Blot (0.1 μg/mL-1 μg/mL)
The optimal working dilution should be determined b

Restrictions

For Research Use only
Format

Liquid

Buffer

In PBS (50 % glycerol, 1 % BSA, 0.09 % sodium azide)

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Target

His Tag

Alternative Name

His-Tag (His Tag Products)

Target Type

Tag