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His Tag antibody (N-Term) (Biotin)

Name: His Tag antibody (N-Term) (Biotin)
SKU: ABIN5579852
Price: 686.67 USD
Availability: InStock

WB, ELISA, IP, FACS, ICC, IHC (p) Host: Rabbit Monoclonal RM146 Biotin

Western Blot analysis of 293T cell lysate Lane 1: transfected and Lane 2: untransfected with a DNA construct encoding His-Tag Histone H3 (G34W) protein using His-Tag monoclonal antibody, clone RM146 (Biotin) at 0.

ELISA

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of naive HepG2 cells (Negative control) (A) and 293T cells expressing His-Tag nuclear protein X (B) with His-Tag monoclonal antibody, clone RM146 (Biotin) .

IHC

3 images

Catalog No. ABIN5579852

Datasheet as PDF

Target See all His Tag Antibodies

His Tag
Binding Specificity
16

2

1
N-Term

Reactivity

Please inquire
Host
93

55

12

9

8

7

2

1
Rabbit
Compatible Secondaries
Clonality
105

69

10
Monoclonal
Conjugate
91

22

14

11

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This His Tag antibody is conjugated to Biotin
Application
152

102

37

27

25

16

14

14

13

13

13

7

7

6

5

5

4

4

3

3

2

2

1

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose

Recombinant rabbit monoclonal antibody raised against His-Tag.

Immunogen

Original antibody is raised against a mixture of synthetic peptides corresponding to N-terminus region of 6X His-Tag and C-terminus region of 6X His-Tag.

Clone

RM146

Isotype

IgG

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Application Notes

ELISA (0.01-0.5 μg/mL)
Flow Cytometry (0.5-2 μg/mL)
Immunocytochemistry (0.5-2 μg/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.1-1 μg/mL)
Immunoprecipitation
Western Blotting (0.1-0.5 μg/mL)
The optimal wor

Restrictions

For Research Use only
Format

Liquid

Buffer

In PBS (50 % glycerol, 1 % BSA, 0.09 % sodium azide)

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Target

His Tag

Alternative Name

His-Tag (His Tag Products)

Target Type

Tag