TBC1D4 antibody (Internal Region, pThr642)

Catalog No. ABIN5596682

Product Details anti-TBC1D4 Antibody

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Binding Specificity: Internal Region, pThr642

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBC1D4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with mouse and human based on 100% sequence homology. Cross-reactivity with TBC1D4 [p Thr642] from other sources has not been determined.

Purification: Anti-TBC1D4 [p Thr642] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Immunogen:

Immunogen: TBC1D4 [p Thr642] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the pT649 site of human TBC1D4 [p Thr642].

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-TBC1D4 pT642 antibody is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~160 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.93 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TBC1D4

Target: TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

Alternative Name: TBC1D4 (TBC1D4 Products)

Synonyms: AS160 antibody, 5930406J04Rik antibody, A930035N22 antibody, AV295684 antibody, As160 antibody, RGD1561609 antibody, TBC1 domain family member 4 antibody, TBC1 domain family, member 4 antibody, TBC1D4 antibody, Tbc1d4 antibody

Background:

Synonyms: TBC1 domain family member 4, Akt substrate of 160 kDa, AS160, TBC1D4, KIAA0603

Background: TBC1D4 [p Thr642] is associated with glucose uptake by promoting insulin-induced glucose transporter. The Akt substrate 160 pT649 activation by Akt has been associated with the GLUT4 translocation upon insulin activation in 3T3-L1 cells. Anti-TBC1D4 [p Thr642] antibody is ideal for investigators interested in Signal Transduction research.

Gene Name: TBC1D4