Calreticulin antibody (C-Term)

Catalog No. ABIN5596697

Product Details anti-Calreticulin Antibody

Target: Calreticulin (CALR)

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calreticulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with human, mouse, rat, primate, and chicken based on 100% sequence homology. Cross-reactivity with Calreticulin precursor from other sources has not been determined.

Purification: Anti-Calreticulin precursor was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Immunogen:

Immunogen: Calreticulin precursor affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human calreticulin precursor protein.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Calreticulin precursor antibody is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~48 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 0.2 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.42 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: Calreticulin precursor (CALR Products)

Synonyms: CRT antibody, RO antibody, SSA antibody, cC1qR antibody, crc antibody, crt antibody, Calregulin antibody, CG9429 antibody, Clr antibody, Dmel\\CG9429 antibody, anon-EST:Liang-2.11 antibody, anon-EST:Liang-2.17 antibody, anon-EST:fe3D7 antibody, clone 2.11 antibody, clone 2.17 antibody, eth antibody, potp antibody, cc1qr antibody, ssa antibody, NV19007 antibody, CRP55 antibody, ERp60 antibody, HACBP antibody, CHOT1 antibody, CHT1 antibody, CT1 antibody, AA589632 antibody, CRTR antibody, Creat antibody, CCDS1 antibody, calrl2 antibody, fc66d10 antibody, wu:fc66d10 antibody, wu:fj50c03 antibody, zgc:66153 antibody, calreticulin antibody, calreticulin L homeolog antibody, Calreticulin antibody, putative calreticulin antibody, solute carrier family 6 member 8 antibody, solute carrier family 6 (neurotransmitter transporter, creatine), member 8 antibody, CALR antibody, calr.L antibody, Crt antibody, crt antibody, CRT antibody, Calr antibody, calr antibody, LMJF_31_2600 antibody, Crc antibody, Slc6a8 antibody, SLC6A8 antibody, LOC8269812 antibody

Background:

Synonyms: CRP55, Calregulin, Endoplasmic reticulum resident protein 60, Calreticulin precursor, CRTC, gpr60

Background: Calreticulin is the major calcium binding protein found in smooth muscle sarcoplasmic reticulum (SR) and non-muscle endoplasmic reticulum (ER) membranes. This protein was originally identified in SR membranes and plays a minor role in calcium storage in skeletal and cardiac muscle SR. Calreticulin is also known as calregulin, CRP55, CaBP3, calsequestrin-like protein and Ro/SS-A antigen. Calreticulin binds calcium with low affinity and high capacity, however it also exhibits a single high affinity binding site. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of calreticulin and other resident ER proteins including glucose regulated protein 78 (GRP78), GRP94 and protein disulfide isomerase (PDI). This sequence is responsible for the retention of newly synthesized proteins within the ER lumen. This retention is reported to be mediated by a KDEL receptor. Recent reports indicate that calreticulin can act as a modulator of the regulation of gene transcription by nuclear hormone receptors and may also act as a molecular chaperone.

Gene Name: CALR

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response