GATA4 antibody (N-Term)
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- Target See all GATA4 Antibodies
- GATA4 (GATA Binding Protein 4 (GATA4))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This GATA4 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Cross-Reactivity (Details)
- A BLAST analysis was used to suggest reactivity with mouse and rat GATA4 based on a 93% homology. Cross-reactivity with GATA4 from other sources has not been determined.
- Purification
- Anti-GATA4 was purified from concentrated tissue culture supernatant Protein A chromatography.
- Immunogen
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Immunogen: GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein correspoding to the first 100 amio acids of human GATA4 protein.
Immunogen Type: Recombinant Protein
- Clone
- 10D8-B10-D4
- Isotype
- IgG1
- Top Product
- Discover our top product GATA4 Primary Antibody
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- Application Notes
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Application Note: Anti-GATA4 antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44 kDa in size corresponding to GATA4 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500-1:1000
ELISA Dilution: 1:2000-1:10,000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- GATA4 (GATA Binding Protein 4 (GATA4))
- Alternative Name
- GATA4 (GATA4 Products)
- Synonyms
- gata-4 antibody, xgata4 antibody, xGATA-4 antibody, MGC81427 antibody, GATA4 antibody, GATA-4 antibody, Gata4 antibody, gata4 antibody, ASD2 antibody, VSD1 antibody, Gata-4 antibody, gata antibody, GATA binding protein 4 antibody, GATA binding protein 4 L homeolog antibody, glutamyl-tRNA(Gln) amidotransferase subunit A antibody, gata4 transcription factor antibody, GATA binding protein 4 S homeolog antibody, GATA4 antibody, gata4.L antibody, gata4 antibody, gatA4 antibody, Gata4 antibody, gata4.S antibody
- Background
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Synonyms: Transcription factor GATA-4, GATA-binding factor 4, GATA-4 Antibody
Background: GATA4, also know as GATA-binding factor 4, acts in cooperation with NKX2-5 as a transcriptional activator of ANF. It binds to the consensus sequence 5'-AGATAG-3' on DNA. GATA4 is critical for the enlargement of cardiac myocytes. Mutations in GATA4 have been linked with congenital heart malfuntions and malformations. Anti-GATA4 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.
Gene Name: GATA4
- Gene ID
- 2626
- UniProt
- P43694
- Pathways
- Peptide Hormone Metabolism, Carbohydrate Homeostasis
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