HDAC8 antibody (C-Term)

Catalog No. ABIN5596727

Product Details anti-HDAC8 Antibody

Target: HDAC8 (Histone Deacetylase 8 (HDAC8))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC8 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with rat, mouse, human, and bovine based on 100% sequence homology. Cross-reactivity with HDAC8 from other sources has not been determined.

Purification: Anti-HDAC8 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Immunogen:

Immunogen: Anti-HDAC8 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the C-terminus of human HDAC8 protein.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-HDAC8 antibody is useful for ELISA, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~41 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1: 10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HDAC8

Target: HDAC8 (Histone Deacetylase 8 (HDAC8))

Alternative Name: HDAC-8 (HDAC8 Products)

Synonyms: HD8 antibody, CDLS5 antibody, HDACL1 antibody, MRXS6 antibody, RPD3 antibody, WTS antibody, 2610007D20Rik antibody, RGD1562895 antibody, wu:fd19a02 antibody, zgc:66196 antibody, ATHDA8 antibody, HDA8 antibody, HISTONE DEACETYLASE 8 antibody, T27G7.14 antibody, T27G7_14 antibody, histone deacetylase 8 antibody, histone deacetylase 8 antibody, histone deacetylase 8 L homeolog antibody, CC1G_05302 antibody, hdac8 antibody, HDAC8 antibody, Hdac8 antibody, hdac8.L antibody, HDA08 antibody

Background:

Synonyms: Histone deacetylase 8, HD8, HDACL1

Background: HDAC8 is located in the nucleus and cytoplasm, expressed weakly in most tissues with higher expression in the heart, brain, kidney, pancreas, liver, lunch, placenta, prostate, and kidney. It is responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). The result of deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Anti-HDAC8 therefore is ideal for investigators interested in Stem Cells , Cardiovascular , or Epigenetics and Nuclear Signaling research.

Gene Name: HDAC8

Pathways: Cellular Glucan Metabolic Process