Phosphothreonine antibody (pThr) (Biotin)

Catalog No. ABIN5596877

Product Details anti-Phosphothreonine Antibody (Biotin)

Target: Phosphothreonine

Binding Specificity: pThr

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phosphothreonine antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Cross-Reactivity (Details): Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphotyrosine or phosphoserine.

Purification: Anti-PhosphoThreonine was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated threonine peptide coupled to agarose followed by conjugation to biotin and subsequent purification. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Biotin.

Immunogen:

Immunogen: PhosphoThreonine affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH.

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: PhosphoThreonine affinity-purified antibody is suitable for use in ELISA and western blotting assays. The antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphoserine residues. The antibody reacts with free phosphothreonine, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine in proteins of both unstimulated and stimulated cell lysates. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.

Western Blot Dilution: 1:500

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:5,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store anti-pT antibody at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Phosphothreonine

Target: Phosphothreonine

Abstract: Phosphothreonine Products

Target Type: Amino Acid

Background:

Synonyms: PhosphoThreonine (pT) reactive antibody PhosphoThreonine (pT) polyclonal antibody, PhosphoThreonine antibody produced in rabbit

Background: Anti-PhosphoThreonine antibodies are broadly reactive allowing these antibodies especially valuable in multiplex analyses and target discovery programs