GFP antibody

Catalog No. ABIN5596890

There is 1 publication for this product available. The Goat Polyclonal anti-GFP antibody is suitable to detect GFP in samples from Aequorea victoria. It has been validated for WB, ELISA, IHC, IF, IP and FM.

Quick Overview for GFP antibody (ABIN5596890)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Goat

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Product Details anti-GFP Antibody

Purpose: GFP Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

Characteristics: Synonyms: goat anti-GFP antibody, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Purification: GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-GFP is designed to detect GFP and its variants. Goat Anti-GFP has been tested by ELISA, Western blot, and immunofluorescence. This product is also suitable for EM, FC, FISH, IHC, IP, purification, and multiplex assays based on published references. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild-type, recombinant, and enhanced forms of GFP. Researchers should determine optimal titers for applications.

Western Blot Dilution: 1:400-1:2,000

Immunoprecipitation Dilution: yes

ELISA Dilution: 1:20,000

IF Microscopy Dilution: 1:500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store GFP antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GFP antibody (ABIN5596890)

Ganjam, Bolte, Matschke, Neitemeier, Dolga, Höllerhage, Höglinger, Adamczyk, Decher, Oertel, Culmsee: "Mitochondrial damage by α-synuclein causes cell death in human dopaminergic neurons." in: Cell death & disease, Vol. 10, Issue 11, pp. 865, (2020) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Background: Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

UniProt: P42212