PAX6 antibody

Catalog No. ABIN5596956

Product Details anti-PAX6 Antibody

Target: PAX6 (Paired Box 6 (PAX6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Cross-Reactivity (Details): Cross-reactivity with PAX6 from other sources has not been determined.

Characteristics:

Sex: Female

Purification: Anti-PAX6 was purified from clarified ascites by Protein A chromatography.

Immunogen:

Immunogen: PAX6 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein correspoding to human PAX6 protein.

Immunogen Type: Recombinant Protein

Clone: 7H10-D3-D4-G5

Isotype: IgG1

Application Details

Application Notes:

Application Note: Anti-PAX6 antibody is suitable for ELISA and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 46.7 kDa in size corresponding to PAX6 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:75,000 - 1:150,000

IF Microscopy Dilution: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Alternative Name: PAX6 (PAX6 Products)

Synonyms: pax6 antibody, an2 antibody, mgda antibody, wagr antibody, pax-6 antibody, pax6a antibody, xpax6 antibody, XLPAX6 antibody, MGC52531 antibody, PAX-6 antibody, AN antibody, AN2 antibody, D11S812E antibody, MGDA antibody, WAGR antibody, Pax-6 antibody, pax6b antibody, 1500038E17Rik antibody, AEY11 antibody, Dey antibody, Gsfaey11 antibody, Sey antibody, PAXZF-A antibody, Pax6.1 antibody, cb280 antibody, fc20e07 antibody, pax-a antibody, pax[zf-a] antibody, paxzfa antibody, wu:fc20e07 antibody, zfpax-6a antibody, zfpax-6b antibody, paired box 6 antibody, paired box 6 L homeolog antibody, paired box 6 S homeolog antibody, paired box 6a antibody, PAX6 antibody, pax6 antibody, Pax6 antibody, pax6.L antibody, pax6.S antibody, pax6a antibody

Background:

Synonyms: Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, AN2, PAX6 Antibody

Background: Paired box protein Pax-6 also known as aniridia type II protein (AN2) or oculorhombin is a transcription factor with key functions in the development of the eye, nose, central nervous system and pancreas. Pax6 further seems to be required for the differentiation of pancreatic islet alpha cells. PAX6 has been shown to bind to a promoter element in the glucagon, insulin and somatostatin promoters and regulates the specification of the ventral neuron subtypes by establishing the correct progenitor domains. Isoform 5a appears to function as a molecular switch that specifies target genes. Anti-PAX6 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

Gene Name: PAX6

Gene ID: 5080

UniProt: P26367

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis