RPA3
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
Biotin
Application Notes
Immunoprecipitation. Western blot. Immunohistochemistry on paraffin sections (5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove fromheat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween(TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
Phosphate buffered saline
Storage
-20 °C
Storage Comment
Store the antibody (in aliquots) at -20 °C to -70 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Background
Replication protein A (RPA) is a single-stranded DNA binding protein. Human RPA is a heterotrimeric protein containing subunits of 70, 32 and 14 kDa. This protein complex is highly conserved in eukaryotes and is essential in DNA replication, homologous recombination and nucleotide excision repair. RPA has been shown to be a target for DNA-dependent protein kinase (DNA-PK) ataxia telangiectasia-mutated gene (ATM) and a cyclin dependent protein kinase.Synonyms: REPA3, RF-A protein 3, RFA3, RP-A p14, Replication factor A protein 3, Replication protein A, Replication protein A 14 kDa subunit