IFT57 antibody (AA 159-193)

Catalog No. ABIN5647795

Product Details anti-IFT57 Antibody

Target: IFT57 (Intraflagellar Transport 57 Homolog (IFT57))

Binding Specificity: AA 159-193

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFT57 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity (Details): Expected species reactivity: Bovine

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 159-193 from the human protein was used as the immunogen for the HIPPI antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the HIPPI antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 1:2000

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the HIPPI antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for IFT57

Target: IFT57 (Intraflagellar Transport 57 Homolog (IFT57))

Alternative Name: HIPPI (IFT57 Products)

Synonyms: Esrrbl1 antibody, 4833420A15Rik antibody, Hippi antibody, MHS4R2 antibody, ESRRBL1 antibody, HIPPI antibody, intraflagellar transport 57 antibody, intraflagellar transport 57 S homeolog antibody, Ift57 antibody, IFT57 antibody, ift57.S antibody

Background: Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro- apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator, however the relevance of such function remains unclear.

UniProt: Q9NWB7

Pathways: Hedgehog Signaling, Positive Regulation of Endopeptidase Activity