MGEA5 antibody (AA 236-269)

Catalog No. ABIN5647928

Product Details anti-MGEA5 Antibody

Target: MGEA5 (Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5))

Binding Specificity: AA 236-269

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MGEA5 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity (Details): Expected species reactivity: Mouse,Rat

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 236-269 from human MGEA5 was used as the immunogen for the MGEA5 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the MGEA5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. WB: 1:1000-1:2000

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the MGEA5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for MGEA5

Target: MGEA5 (Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5))

Alternative Name: MGEA5 (MGEA5 Products)

Synonyms: MEA5 antibody, NCOAT antibody, OGA antibody, 2810009A20Rik antibody, 4833427O07Rik antibody, 5830447M11Rik antibody, AA408215 antibody, Hy5 antibody, Ncoat antibody, mKIAA0679 antibody, O-GLCNACASE antibody, cytochrome c oxidase subunit VIc antibody, meningioma expressed antigen 5 (hyaluronidase) antibody, COX6C antibody, MGEA5 antibody, Mgea5 antibody

Background: Isoform 1 (Cytoplasmic): Cleaves GlcNAc but not GalNAc from O- glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4- methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl- beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:< a href=http://www.uniprot.org/citations/11148210 target=_blank>11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:< a href=http://www.uniprot.org/citations/24088714 target=_blank>24088714).

Isoform 3 (Nuclear): Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1. [UniProt]

UniProt: O60502

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process