COL3 antibody

Catalog No. ABIN5680234

Product Details anti-COL3 Antibody

Target: COL3 (Collagen, Type III (COL3))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL3 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Specificity: Bovine collagen type III 100 % , bovine collagen I <5 %, bovine collagen type II <0.1 %, (solid phase RIA at 1:50 dilution), sheep collagen type III app. 50 % (IHC) Cross-reacting with pig and sheep (IHC on skin and heart tissue)

Cross-Reactivity: Pig (Porcine), Sheep (Ovine)

Purification: Affinity purified

Immunogen: Purified collagen type III from bovine skin

Isotype: IgG

Application Details

Application Notes: IFA, IHC(P), ELISA, RIA , After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended. Positive Control: Bovine skin or liver

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: approx. 1 mg/mL

Buffer: phosphate buffered solution, no BSA and preservative added!

Storage: 4 °C

Target Details for COL3

Target: COL3 (Collagen, Type III (COL3))

Alternative Name: Collagen Type III (COL3 Products)

Synonyms: PT antibody, RPRGL2 antibody, THPH1 antibody, Cf-2 antibody, Cf2 antibody, FII antibody, thrombin antibody, prothrombin antibody, coagulation factor II, thrombin antibody, coagulation factor II antibody, F2 antibody