Western Blotting (WB), ELISA, Immunofluorescence (IF)
Purification
ACAT1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ACAT1 Antibody was raised against synthetic peptide DEEYKRVDFSKVPK found at an internal region of ACAT1.
Verified
ACAT1
Reactivity: Human, Mouse, Rat
WB, ELISA, IF
Host: Goat
Polyclonal
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:2000. Western Blot: Approx 45 kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 45.2 kDa according to Human NP_000010.1, 44.8 kDa according to Mouse NP_659033.1 and 44.7 kDa according to Rat NP_058771.2). Recommended concentration: 0.1-0.3μ, Immunofluorescence:Customer finds particulate cytoplasm staining in HeLa.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
ACAT1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.