Thrombopoietin antibody (Biotin)
-
- Target See all Thrombopoietin (THPO) Antibodies
- Thrombopoietin (THPO)
-
Reactivity
- Human
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- This Thrombopoietin antibody is conjugated to Biotin
-
Application
- Western Blotting (WB), ELISA
- Purification
- Anti-hTPO specific antibody was purified by affinity chromatography and then biotinylated.
- Immunogen
- Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hTPO (human TPO).
- Top Product
- Discover our top product THPO Primary Antibody
-
-
- Application Notes
-
ELISA:
To detect hTPO by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hTPO.
Sandwich:
To detect hTPO by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human TPO (XP-5288) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.
Western Blot:
To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. - Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Storage
- -20 °C
- Storage Comment
- TPO antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
-
- Target
- Thrombopoietin (THPO)
- Alternative Name
- Thrombopoietin (TPO) (THPO Products)
- Synonyms
- MGDF antibody, MKCSF antibody, ML antibody, MPLLG antibody, THCYT1 antibody, TPO antibody, Mgdf antibody, Ml antibody, Mpllg antibody, Tpo antibody, Tpo1 antibody, Tpo2 antibody, Tpo3 antibody, Tpo4 antibody, tpo antibody, LOC100231762 antibody, thrombopoietin antibody, THPO antibody, Thpo antibody, thpo antibody
- Background
- Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
- Gene ID
- 7066
- UniProt
- P40225
- Pathways
- JAK-STAT Signaling, Hormone Activity
-