Thrombopoietin antibody

Catalog No. ABIN5687619

Product Details anti-Thrombopoietin Antibody

Target: Thrombopoietin (THPO)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Thrombopoietin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Purification: Anti-hTPO specific antibody was purified by affinity chromatography employing immobilized hTPO matrix.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hTPO.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTPO (5 ng/mL), a concentration of 4.2 - 6.3 μ,g/mL of this antibody is required.

ELISA:

To detect hTPO by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTPO.

Sandwich:

To detect hTPO by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TPO (XP-5288Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.

Western Blot:
To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: TPO antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Thrombopoietin

Target: Thrombopoietin (THPO)

Alternative Name: Thrombopoietin (TPO) (THPO Products)

Synonyms: MGDF antibody, MKCSF antibody, ML antibody, MPLLG antibody, THCYT1 antibody, TPO antibody, Mgdf antibody, Ml antibody, Mpllg antibody, Tpo antibody, Tpo1 antibody, Tpo2 antibody, Tpo3 antibody, Tpo4 antibody, tpo antibody, LOC100231762 antibody, thrombopoietin antibody, THPO antibody, Thpo antibody, thpo antibody

Background: Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Gene ID: 7066

UniProt: P40225

Pathways: JAK-STAT Signaling, Hormone Activity