This antibody is expected to recognize all reported isoform 1 (NP_001659.1) only.
Purification
ARNT Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ARNT Antibody was raised against synthetic peptide CNDKERFARSDDEQS found at an internal region (near the N Terminus) of ARNT.
ELISA: antibody detection limit dilution 1:4000. Western Blot: Approx 90 kDa band observed in nuclear lysates of cell lines HeLa and HepG2 (calculated MW of 86.6 kDa according to NP_001659.1). Recommended concentration: 2-4μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
ARNT is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.