ATP5B Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ATP5B Antibody was raised against synthetic peptide EPIDERGPIKTKQ found at an internal region of ATP5B.
ELISA: antibody detection limit dilution 1:4000. Western Blot: Approx 55 kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 56.6 kDa according to NP_001677.2). Recommended concentration: 0.3-1μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
ATP5B is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.