BCAR1 antibody (Internal Region)

Catalog No. ABIN5687684

Product Details anti-BCAR1 Antibody

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This BCAR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody is expected to recognize reported isoforms1, 2, 3, 5, 6 and 7.

Purification: BCAR1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: BCAR1 Antibody was raised against synthetic peptide KQTSTFSKQTPHH found at an internal region of BCAR1.

Application Details

Application Notes: ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 100  kDa band observed in lysates of Human breast cancer and of cell line MCF7 (calculated MW of 97.9  kDa according to NP_001164185.1). Recommended concentration: 1-3μ,g/mL. Immunohistochemistry: In paraffin embedded Human Uterus shows strong staining at the glandular side of the endometrial epithelium. Recommended concentration, 5-10μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: BCAR1 is supplied in Tris saline, 0.02 % sodium azide,  pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C. Minimize freezing and thawing.

Target Details for BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Alternative Name: BCAR1 (BCAR1 Products)

Synonyms: CAS antibody, CAS1 antibody, CASS1 antibody, CRKAS antibody, P130Cas antibody, AI385681 antibody, Cas antibody, Crkas antibody, P130CAS antibody, MGC68466 antibody, cas antibody, p130cas antibody, BCAR1 antibody, fc17f11 antibody, wu:fc17f11 antibody, zgc:175192 antibody, BCAR1, Cas family scaffolding protein antibody, breast cancer anti-estrogen resistance 1 antibody, breast cancer anti-estrogen resistance 1 L homeolog antibody, breast cancer anti-estrogen resistance protein 1 antibody, BCAR1 antibody, Bcar1 antibody, bcar1.L antibody, bcar1 antibody, LOC100435586 antibody

Molecular Weight: Approx 100kDa

Gene ID: 9564

NCBI Accession: NP_001164185, NP_001164186, NP_001164187, NP_001164188, NP_001164189, NP_055382, NP_001164190, NP_001164191, NP_001164192

UniProt: P56945

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling