This antibody is expected to recognize both reported isoforms (NP_005889.3, NP_976240.1).
Purification
CAPRIN1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
CAPRIN1 Antibody was raised against synthetic peptide EQRPQKEPIDQ found at an internal region of CAPRIN1.
Caprin-1
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 80+140 kDa bands observed in lysates of cell line Caco-2, HeLa, Jurkat and K562 (calculated MW of 78.4 kDa according to NP_005889.3). The ~140 kDa band corresponds to the reported p137 (Ellis and Luzio, J Biol Chem. 1995 Sep 1,270(35):2
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
CAPRIN1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.
Target
Caprin-1
(Cell Cycle Associated Protein 1 (Caprin-1))