Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
This antibody is expected to recognize both reported isoforms (NP_002128.1, NP_112533.1).
Purification
HNRNPA2B1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
HNRNPA2B1 Antibody was raised against synthetic peptide DTIEIITDRQS found at an internal region of HNRNPA2B1.
HNRNPA2B1
Reactivity: Human, Mouse, Rat, Pig, Dog, Monkey
WB, IHC, IF, IC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:64000. Western Blot: Approx 36 kDa band observed in lysates of cell lines HeLa and MCF7 and in nuclear lysates of cell line NIH3T3 (calculated MW of 36.0 kDa according to NP_002128.1). Recommended concentration: 1-3μ,g/mL. Immunohistochemistry: In paraffin embedded Human Small Intestine shows nuclear staining. Recommended concentration, 5-10μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
HNRNPA2B1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.