TNNT3 antibody (C-Term)

Catalog No. ABIN5688298

Product Details anti-TNNT3 Antibody

Target: TNNT3 (Fast Skeletal Troponin T (TNNT3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This TNNT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody is expected to recognize isoform 1, 2 and 4 (NP_006748.1, NP_001036246.1, NP_001036247.1).

Purification: TNNT3 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: TNNT3 Antibody was raised against synthetic peptide TTLRSRIDQAQKHSK found near the C Terminus of TNNT3.

Application Details

Application Notes: ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 35  kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 30.6  kDa according to NP_006748.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Marian et Immunohistochemistry: In paraffin embedded Human Skeletal Muscle shows staining of select fibrils. Recommended concentration, 4-8μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: TNNT3 is supplied in Tris saline, 0.02 % sodium azide,  pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C. Minimize freezing and thawing.

Target Details for TNNT3

Target: TNNT3 (Fast Skeletal Troponin T (TNNT3))

Alternative Name: TNNT3 (TNNT3 Products)

Synonyms: fTnT antibody, tnnt3a antibody, TNTF antibody, TnTf antibody, Tnt antibody, Tnnt3 antibody, troponin T3, fast skeletal type antibody, troponin T3, skeletal, fast antibody, troponin T3, fast skeletal type S homeolog antibody, troponin T type 3 (skeletal, fast) antibody, troponin T1, slow skeletal type antibody, TNNT3 antibody, Tnnt3 antibody, tnnt3.S antibody, TNNT1 antibody

Molecular Weight: Approx 35kDa

Gene ID: 7140

NCBI Accession: NP_006748, NP_001036246, NP_001036247

UniProt: P45378