FGF23
Reactivity: Human
ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: (direct and indirect: 1:2,000-1:5,000). Immunohistochemistry: (paraffin sections (1:100-1:500)). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05 % Tween 20 and 5 % skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Preservative
Without preservative
Storage
-20 °C
Storage Comment
Stable for at least 1 year after receipt when stored at -20°C.
FGF-23 is a regulator of phosphate homeostasis. It upregulates EGR1 expression in the presence of KLBy. Acts directly on the parathyroid to decrease PTH secretion. Regulates the vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF-23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) and of hyperphosphatemic familial tumoral calcinosis (HFTC).