Midkine antibody (Biotin)

Catalog No. ABIN5689076

Product Details anti-Midkine Antibody (Biotin)

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Midkine antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Purification: Anti-hMidkine specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant (human Midkine).

Application Details

Application Notes: ELISA:

To detect hMidkine by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hMidkine.

Sandwich:

To detect hMidkine by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human Midkine (XP-5230) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMidkine.

Western Blot:
To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMidkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: Midkine antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Midkine

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Alternative Name: Midkine (MDK Products)

Synonyms: ARAP antibody, MK antibody, NEGF2 antibody, Mek antibody, MK-B antibody, PTF-alpha-1 antibody, mdk antibody, midkine antibody, negf2 antibody, RIHB antibody, cb518 antibody, fb79b05 antibody, fj33b02 antibody, fj48a12 antibody, mdk2 antibody, plei2 antibody, wu:fb79b05 antibody, wu:fj33b02 antibody, wu:fj48a12 antibody, MK-A antibody, PTF-alpha-2 antibody, X-PTF-alpha1 antibody, XMK antibody, mdk-a antibody, mdk-b antibody, midkine antibody, midkine L homeolog antibody, midkine (neurite growth-promoting factor 2) antibody, midkine b antibody, midkine S homeolog antibody, MDK antibody, Mdk antibody, mdk.L antibody, mdkb antibody, mdk.S antibody

Background: Midkine exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Its expression is predominant only for a short period from approximately one-half to two-thirds of the way through gestation, before and after that, it is barely detectable. Midkine was first found in differentiating mouse teratocarcinoma cells. It has neurotrophic activities and is mitogenic to certain, but not to all, fibroblast cell lines.

Gene ID: 4192

UniProt: P21741

Pathways: RTK Signaling, M Phase, Skeletal Muscle Fiber Development