HDAC4/HDAC5/HDAC9 antibody (pSer220, pSer246, pSer259)

Catalog No. ABIN5689653

Product Details anti-HDAC4/HDAC5/HDAC9 Antibody

Target: HDAC4/HDAC5/HDAC9 (HDAC4,HDAC5,HDAC9)

Binding Specificity: pSer220, pSer246, pSer259

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC4/HDAC5/HDAC9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: This antibody detects endogenous level of HDAC4/HDAC5/HDAC9 only when phosphorylated at serine 246/259/220.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Immunogen: HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) antibody was raised against a peptide sequence around phosphorylation site of serine 246/259/220 (T-A-S (p) -EP) derived from Human HDAC4/HDAC5/HDAC9.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for HDAC4/HDAC5/HDAC9

Target: HDAC4/HDAC5/HDAC9 (HDAC4,HDAC5,HDAC9)

Alternative Name: HDAC4 / HDAC5 / HDAC9 (HDAC4/HDAC5/HDAC9 Products)

Synonyms: AHO3 antibody, BDMR antibody, HA6116 antibody, HD4 antibody, HDAC-4 antibody, HDAC-A antibody, HDACA antibody, histone deacetylase 4 antibody, HDAC4 antibody

Background: Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general, they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs, classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions (for example HDAC-14.3.3 complexes are retained in the cytosol) and by the class to which they belong (class 1 HDACs are predominantly nuclear whilst class 2 HDACs shuttle between the nucleus and cytosol). HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents.

Molecular Weight: 140, 124 kDa

Gene ID: 9759, 10014, 9734

NCBI Accession: NP_006028, NP_001015053, NP_055522

UniProt: P56524, Q9UQL6, Q9UKV0