EIF2A antibody (pSer51)
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- Target See all EIF2A Antibodies
- EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))
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Binding Specificity
- pSer51
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This EIF2A antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Specificity
- This antibody detects endogenous level of eIF2α, only when phosphorylated at serine 51.
- Purification
- Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
- Immunogen
- eIF2a (Phospho-Ser51) antibody was raised against a peptide sequence around phosphorylation site of serine 51 (E-L-S (p) -R-R) derived from Human eIF2α,.
- Top Product
- Discover our top product EIF2A Primary Antibody
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- Application Notes
- Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store antibody at -20°C for up to one year.
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- Target
- EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))
- Alternative Name
- eIF2a (EIF2A Products)
- Synonyms
- ATEIF2-A2 antibody, EIF2-A2 antibody, eukaryotic translation initiation factor 2 alpha subunit antibody, EIF-2A antibody, MST089 antibody, MSTP004 antibody, MSTP089 antibody, D030048D22 antibody, D3Ertd194e antibody, im:7145971 antibody, zgc:110460 antibody, DDBDRAFT_0185936 antibody, DDBDRAFT_0234177 antibody, DDB_0185936 antibody, DDB_0234177 antibody, Eif2a antibody, eif2a antibody, eif2alpha antibody, EIF2A antibody, CG9946 antibody, Dmel\\CG9946 antibody, Eif2alpha antibody, IF2A_DROME antibody, M(1)14C antibody, M(1)19-153 antibody, M(1)8e3-10 antibody, deIF2alpha antibody, eIF-2 antibody, eIF-2 a antibody, eIF-2 alpha antibody, eIF2 alpha antibody, eIF2-alpha antibody, eIF2a antibody, eIF2alpha antibody, eiF-2alpha antibody, elF2alpha antibody, l(1)14Cf antibody, l(1)19-153 antibody, eukaryotic translation initiation factor 2 alpha subunit antibody, eukaryotic translation initiation factor 2A antibody, eukaryotic translation initiation factor 2A, 65kDa antibody, eukaryotic translation initiation factor 2A S homeolog antibody, eukaryotic translation initiation factor antibody, eukaryotic translation Initiation Factor 2alpha antibody, EIF2 ALPHA antibody, EIF2A antibody, Eif2a antibody, eif2a antibody, eIF2a antibody, eif2a.S antibody, eif2A antibody, eIF-2alpha antibody
- Background
- Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
- Molecular Weight
- 38 kDa
- Gene ID
- 1965
- NCBI Accession
- NP_004085
- UniProt
- P05198
- Pathways
- Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly
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