alpha Actinin 4 antibody (AA 27-38)

Catalog No. ABIN5689869

Product Details anti-alpha Actinin 4 Antibody

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Binding Specificity: AA 27-38

Reactivity: Human, Mouse, Rat, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Actinin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Specificity: Recognizes human, mouse, rat, pig and Chinese hamster actinin-4. Does not cross-react with actinin-1, -2 or -3.

Purification: Antigen affinity purified.

Immunogen: Synthetic peptide corresponding to aa 27-38 of the N-terminal alpha-Actinin-4, coupled via a C-terminal cysteine residue to KLH.

Application Details

Application Notes: Immunocytochemistry: (1:250- 1:1,600) . Immunohistochemistry: (1:250- 1:1,600) . Immunoprecipitation: (1:10) . Western Blot: (1:1,000-1:2,500) . Optimal conditions must be determined individually for each application.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Liquid. In PBS containing 1 mg/mL BSA and 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for at least 1 year after receipt when stored at -20°C.

Target Details for alpha Actinin 4

Target: alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))

Alternative Name: alpha-Actinin-4 (ACTN4 Products)

Synonyms: fsgs antibody, fsgs1 antibody, C77391 antibody, ACTININ-4 antibody, FSGS antibody, FSGS1 antibody, wu:fb53f05 antibody, zgc:63508 antibody, actinin alpha 4 antibody, actinin, alpha 4 antibody, actinin alpha 4 S homeolog antibody, actn4 antibody, ACTN4 antibody, Actn4 antibody, actn4.S antibody

Background: Alpha-Actinin 4 is an actin-bundling protein of ~100  kDa that is associated with cell motility, endocytosis and cancer invasion. The alpha-actinin family comprises two non-muscle isoforms (alpha-actinin-1 and -4) and two skeletal muscle isoforms (alpha-actinin-2 and -3), with alpha-actinin-2 being also expressed in cardiac muscle. While alpha-actinin-4 is almost ubiquitously expressed, particularly high concentrations are found in glomeruli. On the subcellular level it is associated with actin stress fibers, but in certain cells it also localizes to the nucleus. Mutations in the alpha-actinin-4 gene cause an autosomal-dominant form of familial focal segmental glomerulosclerosis (FSGS), which is thought to result from a defect in glomerular podocyte function. A point mutation in the alpha-actinin-4 gene was found to generate an antigenic peptide that is recognized by autologous cytolytic T lymphocytes (CTL) on a human lung carcinoma. alpha-Actinin-4 interacts with a variety of proteins, including the ring finger protein BERP, the PDZ-LIM protein CLP-36, the hemidesmosomal and cell-cell contact protein BP180, and the tight junction protein MAGI-1. Moreover, alpha-actinin-4 forms a ternary complex with Ca2+/Calmodulin-dependent protein kinase II and densin-180, a protein of postsynaptic densities in CNS neurons. Ca2+-dependent association of alpha-actinin-4 with E3KARP is required for Ca2+-dependent inhibition of the Na+/H+ exchanger 3 (NHE3).

Gene ID: 81

NCBI Accession: NP_004915

UniProt: O43707

Pathways: Proton Transport