HDAC2 antibody is human, mouse and rat reactive. At least two isoforms of HDAC2 are known to exist, this antibody will detect both isoforms. HDAC2 antibody is predicted to not cross-react with other members of the HDAC family.
Purification
HDAC2 antibody is affinity chromatography purified via peptide column.
Immunogen
HDAC2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human HDAC2.
The immunogen is located within the last 50 amino acids of HDAC2.
HDAC2 antibody can be used for detection of HDAC2 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
HDAC2 antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
HDAC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
The histone deacetylase (HDAC) family contains multiple members which are divided into four classes. Class I of the HDAC family comprises four members, HDAC1, 2, 3, and 8, each of which contains a deacetylase domain and exhibits a different, individual substrate specificity and function in vivo (1). HDAC2 was first identified as as a mammalian homolog to the yeast transcriptional regulator RPD3 (2). HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor (2,3). Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events (4).