IL1RN antibody

Catalog No. ABIN5690005

Product Details anti-IL1RN Antibody

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1RN antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Purification: IL-1RA specific antibody was purified by affinity chromatography employing an immobilized Human IL-1RA matrix.

Immunogen: E.coli derived Recombinant Human IL-1Ra

Application Details

Application Notes: ELISA:
Sandwich:
To detect Human IL-1RA by sandwich ELISA (using 100μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Polyclonal Biotinylated Anti-Human IL-1RA (38-139) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-1RA.

Western Blot:

To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Neutralization:

To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-1RA (50.0 ng/mL) and Human IL-1a(50.0 pg/mL), a concentration of 2.5-3.7 μ,g/mL of this antibody is required.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL1RN

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Alternative Name: IL-1RA (IL1RN Products)

Synonyms: DIRA antibody, ICIL-1RA antibody, IL-1RN antibody, IL-1ra antibody, IL-1ra3 antibody, IL1F3 antibody, IL1RA antibody, IRAP antibody, MVCD4 antibody, IRAP1 antibody, IL1RN antibody, IL-1RA antibody, F630041P17Rik antibody, interleukin 1 receptor antagonist antibody, interleukin 1 receptor antagonist-like antibody, IL1RN antibody, Il1rn antibody, IL1RNL antibody

Gene ID: 3557

UniProt: P18510

Pathways: NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints