OLIG2 antibody (AA 80-130)

Catalog No. ABIN5690088

Product Details anti-OLIG2 Antibody

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Binding Specificity: AA 80-130

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OLIG2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: OLIG2 antibody is human, mouse and rat reactive. It is predicted to not cross-react with other members of the OLIG family of proteins. At least three isoforms of OLIG2 are known to exist, this antibody will detect all three isoforms.

Purification: OLIG2 antibody is affinity chromatography purified via peptide column.

Immunogen: OLIG2 antibody was raised against a 15 amino acid peptide near the amino terminus of human OLIG2.

The immunogen is located within amino acids 80 - 130 of OLIG2.

Isotype: IgG

Application Details

Application Notes: OLIG2 antibody can be used for detection of OLIG2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: OLIG2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: OLIG2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for OLIG2

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Alternative Name: OLIG2 (OLIG2 Products)

Synonyms: olig3.2 antibody, MGC89336 antibody, OLIG2 antibody, BHLHB1 antibody, OLIGO2 antibody, PRKCBP2 antibody, RACK17 antibody, bHLHe19 antibody, wu:fc64g09 antibody, AI604895 antibody, Bhlhb1 antibody, Olg-2 antibody, Oligo2 antibody, RK17 antibody, oligodendrocyte transcription factor 4 antibody, oligodendrocyte transcription factor 2 antibody, oligodendrocyte lineage transcription factor 2 antibody, olig4 antibody, OLIG2 antibody, Olig2 antibody, olig2 antibody

Background: The oligodendrocyte transcription factors 1 and 2 (OLIG1 and OLIG2, respectively) make up part of basic helix-loop-helix (bHLH) family of transcription factors that are specifically expressed in zones of the neuroepithelium from which oligodendrocyte precursors emerge (1). Both OLIG1 and OLIG2 genes are downstream targets of Sonic hedgehog and are expressed exclusively in the central nervous system (2). OLIG2 is first observed in the ventral most p3 progenitor domain of the ventral neural tube while OLIG1 is first expressed in the dorsal portion of the p3 domain (2). Mice overexpressing OLIG2 exhibit impaired potassium channel expression in neural progenitors and proliferation of these cells similar to that seen in Down Syndrome, suggesting that OLIG2 may play a role in this pathology (3).

Molecular Weight: Predicted: 36 kDa

Observed: 37 kDa

Gene ID: 10215

NCBI Accession: NP_005797

UniProt: Q13516