Betacellulin antibody

Catalog No. ABIN5690378

Product Details anti-Betacellulin Antibody

Target: Betacellulin (BTC)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Betacellulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBetacellulin. Human Betacellulin specific antibody was purified by affinity chromatography employing immobilized hBetacellulin matrix.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human Betacellulin (0.1 ng/mL), a concentration of ≤ 0.1 μ,g/mL of this antibody is required.

ELISA:
Indirect:
To detect hBetacellulin by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin.

Sandwich
To detect hBetacellulin by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Betacellulin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBetacellulin.

Western Blot:
To detect hBetacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBetacellulin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: Betacellulin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Betacellulin

Target: Betacellulin (BTC)

Alternative Name: Betacellulin (BTC Products)

Synonyms: BTC antibody, LOC100228121 antibody, probetacellulin antibody, Bcn antibody, betacellulin antibody, betacellulin, epidermal growth factor family member antibody, BTC antibody, LOC100228121 antibody, Btc antibody

Background: Betacellulin (BTC) is a heparin-binding member of the EGF family of growth factors that includes epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), amphiregulin (AR), heparin-binding EGF-like growth factor (HB-EGF), and the various heregulins (HRG).

Gene ID: 685

UniProt: P35070

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway