IL-21 antibody

Catalog No. ABIN5690418

Product Details anti-IL-21 Antibody

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-21 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP7. Human IGF-BP7 specific antibody was purified by affinity chromatography employing immobilized hIGF-BP7 matrix.

Application Details

Application Notes: ELISA:
Indirect:
To detect hIL-21 by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-21.

Sandwich:

To detect hIL-21 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Human IL-21 (38-134) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-21.

Western Blot:
To detect hIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL-21

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: IL-21 (IL21 Products)

Synonyms: IL-21 antibody, Za11 antibody, interleukin 21 antibody, IL21 antibody, Il21 antibody

Gene ID: 59067

UniProt: Q9HBE4

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response