LGALS1/Galectin 1 antibody (Biotin)

Catalog No. ABIN5690519

Product Details anti-LGALS1/Galectin 1 Antibody (Biotin)

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LGALS1/Galectin 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGalectin-1. Human Galectin-1 specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hGalectin-1 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGalectin-1

Sandwich
To detect hGalectin-1 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human Galectin-1 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGalectin-1.

Western Blot:
To detect hGalectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: Galectin-1 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for LGALS1/Galectin 1

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Alternative Name: Galectin-1 (LGALS1 Products)

Synonyms: GAL1 antibody, GBP antibody, Galectin-1 antibody, LGAS1 antibody, LGALS1 antibody, MGC64502 antibody, GB10026 antibody, AA410090 antibody, Gal-1 antibody, Galbp antibody, L-14.5 antibody, L14 antibody, Lect14 antibody, galectin-1 antibody, Galaptin antibody, galectin 1 antibody, galectin-1 antibody, lectin, galactoside-binding, soluble, 1 L homeolog antibody, protein enabled antibody, lectin, galactose binding, soluble 1 antibody, LGALS1 antibody, Lgals1 antibody, CC1G_05003 antibody, CC1G_05004 antibody, lgals1.L antibody, LOC408848 antibody

Background: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin 1 may act as an autocrine negative growth factor that regulates cell proliferation. It also may regulate cell apoptosis and cell differentiation. It binds beta-galactoside, CD45, CD3 and CD4. Galectin 1 inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. It is found in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney, placenta and hematopoietic cells.

Gene ID: 3956

UniProt: P09382

Pathways: Carbohydrate Homeostasis