Galectin 3 antibody (Biotin)

Catalog No. ABIN5690521

Product Details anti-Galectin 3 Antibody (Biotin)

Target: Galectin 3 (LGALS3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Galectin 3 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Galectin-3. Human Galectin-3 specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect Galectin-3 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Galectin-3.

Sandwich
To detect Galectin-3 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human Galectin-3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Galectin-3.

Western Blot:
To detect Galectin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Galectin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: Galectin-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Galectin 3

Target: Galectin 3 (LGALS3)

Alternative Name: Galectin-3 (LGALS3 Products)

Synonyms: CBP 35 antibody, Gal-3 antibody, Galectin-3 antibody, leg3 antibody, galactoside-binding antibody, galectin-3 antibody, lectin antibody, soluble antibody, CBP35 antibody, GAL3 antibody, GALBP antibody, GALIG antibody, L31 antibody, LGALS2 antibody, MAC2 antibody, MGC52947 antibody, MGC75803 antibody, LGALS3 antibody, gal-3 antibody, GBP antibody, L-34 antibody, Mac-2 antibody, gal3 antibody, galectin 3 antibody, Galectin-3 antibody, avian beta-defensin 3 antibody, lectin, galactoside-binding, soluble, 3 L homeolog antibody, lectin, galactoside-binding, soluble, 3 antibody, galectin 2 antibody, lectin, galactose binding, soluble 3 antibody, LGALS3 antibody, leg3 antibody, AvBD3 antibody, lgals3.L antibody, lgals3 antibody, LGALS2 antibody, Lgals3 antibody

Target Type: Chemical

Background: Galectins are a new family of animal lectins which appear to exhibit a variety of biological functions. Lectins, of either plant or animal origin, are carbohydrate binding proteins that interact with glycoprotein and glycolipids on the surface of animal cells.

Gene ID: 3958

UniProt: P17931

Pathways: RTK Signaling