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TNC antibody

TNC Reactivity: Human WB, IP, IHC (p), ICC, IHC (fro) Host: Mouse Monoclonal T2H5 unconjugated
Catalog No. ABIN5691113
  • Target See all TNC Antibodies
    TNC (Tenascin C (TNC))
    Reactivity
    • 75
    • 43
    • 37
    • 15
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 68
    • 29
    • 2
    • 2
    • 1
    Mouse
    Clonality
    • 63
    • 39
    Monoclonal
    Conjugate
    • 44
    • 11
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TNC antibody is un-conjugated
    Application
    • 70
    • 26
    • 26
    • 25
    • 23
    • 14
    • 12
    • 11
    • 11
    • 9
    • 9
    • 7
    • 4
    • 3
    • 2
    • 2
    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    In immunoblotting assays on an extract of a human osteosarcoma cell line (U2OS) T2H5 recognizes a 265 kDa protein. Tenascin C contains an exstensive number of potential glycosylation sites, which may explain its migration at a higher molecular weight than predicted. Human tonsilar tissue can be used as a positive control in immunocytochemistry and immunohistochemistry.
    Immunogen
    T2H5 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a BALB/c mouse immunized with full length native tenascin protein purified from a homogenate of a human mammary tumour specimen.
    Clone
    T2H5
    Isotype
    IgG1
    Top Product
    Discover our top product TNC Primary Antibody
  • Application Notes
    T2H5 is suitable for immunoblotting, immunocytochemistry, and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
    Restrictions
    For Research Use only
  • Buffer
    Each vial contains 100 μL 1 mg/mL purified monoclonal antibody in PBS containing 0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C, or in small aliquots at -20°C.
  • Target
    TNC (Tenascin C (TNC))
    Alternative Name
    Tenascin C (TNC Products)
    Synonyms
    tn antibody, 150-225 antibody, GMEM antibody, GP antibody, HXB antibody, JI antibody, TN antibody, TN-C antibody, AI528729 antibody, C130033P17Rik antibody, Hxb antibody, Ten antibody, cytotactin antibody, tenascin-C antibody, P230 antibody, tenc antibody, wu:fk04d02 antibody, tenascin C L homeolog antibody, tenascin C antibody, tnc.L antibody, TNC antibody, MCYG_00667 antibody, tnc antibody, Tnc antibody
    Background
    Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development. It has been described under a variety of names, i.e. cytotactin, hexabrachion protein, J1, myotendinous antigen and glioma mesenchymal extracellular matrix. In undifferentiated tumors it is also detectable in the neovasculature and stroma. The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. For example, this extracellular matrix protein is implicated in guidance of migrating neurons as well as axons during neural development as well as neuronal regeneration. In in vitro studies it promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. The cellular ligands for tenascin include integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
    Pathways
    Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development
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