GAPDH antibody

Catalog No. ABIN5700902

Product Details anti-GAPDH Antibody

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Immunogen: glyceraldehyde-3-phosphate dehydrogenase

Isotype: IgG1

Application Details

Application Notes: WB : 1:5000-1:20000 IP : 1:3000-1:20000 IHC : 1:50-1:500 IF : 1:20-1:200

Comment:

HeLa cells were subjected to SDS PAGE followed by western blot with FNab03343 (GAPDH antibody) at dilution of 1:10000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH (GAPDH Products)

Synonyms: G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody

Background: Synonyms:G3PD, GAPD, GAPDH Background:Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules(By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate(G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT(gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs(such as ceruplasmin) and suppresses their translation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues.

Molecular Weight: 36 kDa

Gene ID: 2597

UniProt: P04406