USP13 antibody

Catalog No. ABIN5701380

Product Details anti-USP13 Antibody

Target: USP13 (Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Immunogen: ubiquitin specific peptidase 13 (isopeptidase T-3)

Isotype: IgG

Application Details

Application Notes: WB : 1:500-1:5000,IHC:1:20-1:200,IP:1:200-1:2000

Comment:

HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab09308(USP13 antibody) at dilution of 1:400

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for USP13

Target: USP13 (Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13))

Alternative Name: USP13 (USP13 Products)

Synonyms: ISOT3 antibody, IsoT-3 antibody, 2700071E21Rik antibody, AI848077 antibody, usp13 antibody, MGC163140 antibody, ubiquitin specific peptidase 13 antibody, ubiquitin specific peptidase 13 (isopeptidase T-3) antibody, ubiquitin carboxyl-terminal hydrolase 13 antibody, universal stress protein antibody, USP13 antibody, Usp13 antibody, usp13 antibody, MGYG_01656 antibody, RR_RS02895 antibody

Background: Synonyms:Deubiquitinating enzyme 13, Isopeptidase T 3, IsoT 3, ISOT3, Ubiquitin thiolesterase 13, USP13 Background:Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation(ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation(ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro, however, additional experiments are required to confirm such data.

Molecular Weight: 43kDa

Gene ID: 8975

UniProt: Q92995

Pathways: SARS-CoV-2 Protein Interactome