AKAP8L antibody

Catalog No. ABIN5702022

Product Details anti-AKAP8L Antibody

Target: AKAP8L (A Kinase (PRKA) Anchor Protein 8-Like (AKAP8L))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP8L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Immunogen: A kinase (PRKA) anchor protein 8-like

Isotype: IgG

Application Details

Application Notes: WB :1:200-1:2000,IF:1:20-200

Comment:

PC-3 cells were subjected to SDS PAGE followed by western blot with FNab00258( AKAP8L Antibody) at dilution of 1:300

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for AKAP8L

Target: AKAP8L (A Kinase (PRKA) Anchor Protein 8-Like (AKAP8L))

Alternative Name: AKAP8L (AKAP8L Products)

Synonyms: HA95 antibody, HAP95 antibody, NAKAP antibody, NAKAP95 antibody, Nakap95 antibody, zgc:85966 antibody, AKAP8L antibody, A-kinase anchoring protein 8 like antibody, A kinase (PRKA) anchor protein 8-like antibody, A-kinase anchoring protein 8 like L homeolog antibody, AKAP8L antibody, Akap8l antibody, akap8l antibody, akap8l.L antibody

Background: Synonyms:A kinase anchor protein 8 like, AKAP8 like protein, AKAP8L, DKFZp434L0650, HA95, HAP95, Helicase A binding protein 95, Homologous to AKAP95 protein, NAKAP, NAKAP95, Neighbor of AKAP95 Background:Could play a role in constitutive transport element(CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export(PubMed:10748171, PubMed:11402034). Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA(PubMed:10761695, PubMed:11884601). May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis(PubMed:11034899). May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta(PubMed:12538639). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3, in this function seems to act redundantly with AKAP8(PubMed:16980585). May be involved in regulation of pre-mRNA splicing(PubMed:17594903). (Microbial infection) In case of EBV infection, may target PRKACA to EBNA-LP-containing nuclear sites to modulate transcription from specific promoters(PubMed:11884601). Can synergize with DHX9 to activate the CTE-mediated gene expression of type D retroviruses(PubMed:11402034). In case of HIV-1 infection, involved in the DHX9-promoted annealing of host tRNA(Lys3) to viral genomic RNA as a primer in reverse transcription, in vitro negatively regulates DHX9 annealing activity(PubMed:25034436).

Molecular Weight: 72kDa

Gene ID: 26993

UniProt: Q9ULX6

Pathways: SARS-CoV-2 Protein Interactome