PKM2 antibody (Internal Region)
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- Target See all PKM2 Antibodies
- PKM2 (Pyruvate Kinase M2 (PKM2))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PKM2 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Characteristics
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Synonyms: rabbit anti-PKM2 antibody, PKM 2, PKM-2, Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58, OIP3, OIP-3, PK2, PK3
Background: Anti-PKM2 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Pyruvate kinase has been shown to be expressed in tumor, embryonic and normal proliferating cells in varying levels. Studies show that when PKM2 is secreted it can induce EGFR phosphorylation which stimulates the EGFR downstream pathway. Previously PKM has been shown to be critical for aerobic glycolysis. Four isoforms of PKM play alternative roles within the system and post-translational modifications along these isoforms induce multiple pathways making this Anti-PKM2 antibody ideal for investigators involved in cancer research and post-translational modifications.
Gene Name: PKM
- Purification
- Anti-PKM2 is directed against human PKM2 at an internal position. This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads. A BLAST analysis was used to suggest reactivity with this protein in African frog and rat based on 100% homology and rabbit and mouse based on 93.3% homology for the immunogen sequence.
- Immunogen
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Immunogen: Affinity purified Anti-PKM2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human PKM2 protein.
Immunogen Type: Peptide
- Isotype
- IgG
- Top Product
- Discover our top product PKM2 Primary Antibody
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- Application Notes
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Application Note: This affinity purified antibody has been tested for use in western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band 56-58 kDa in size corresponding to PKM2 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 5 μg/mL
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Expiry Date
- 12 months
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- Target
- PKM2 (Pyruvate Kinase M2 (PKM2))
- Alternative Name
- PKM2 (PKM2 Products)
- Synonyms
- CTHBP antibody, OIP3 antibody, PK3 antibody, PKM2 antibody, TCB antibody, THBP1 antibody, AA414905 antibody, AL024370 antibody, AL024424 antibody, Pk-2 antibody, Pk-3 antibody, Pk3 antibody, Pkm2 antibody, PKM12 antibody, PKM antibody, pyruvate kinase M1/2 antibody, pyruvate kinase, muscle antibody, PKM antibody, Pkm antibody
- Gene ID
- 5315
- NCBI Accession
- NP_001193725
- UniProt
- P14618
- Pathways
- Warburg Effect
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