PIK3CB antibody (Catalytic Subunit beta, N-Term)

Catalog No. ABIN5706834

Product Details anti-PIK3CB Antibody

Target: PIK3CB (Phosphoinositide-3-Kinase, Catalytic, beta Polypeptide (PIK3CB))

Binding Specificity: Catalytic Subunit beta, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3CB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Characteristics:

Synonyms: rabbit anti-PIK3CB antibody, rabbit anti-PI3K beta antibody, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform, PI3-kinase subunit beta, PI3K-ß, PI3Kbeta, PtdIns-3-kinase subunit beta, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta, PtdIns-3-kinase subunit p110-beta, p110beta, PIK3C1

Background: PIK3CB is a catalytic subunit of phosphoinositide-3-kinase beta. PIK3CB catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration and cell survival. PTEN reverses this process. PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN. Furthermore, PI3KCB down regulation can suppress cell growth in malignant gliomas. Anti-PIK3C beta Antibody is suitable for researcher in Cancer, Immunology and Nuclear Signaling research.

Gene Name: PIK3CB

Purification: Anti-PIK3C beta was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human PIK3C beta protein.  A BLAST analysis was used to suggest cross-reactivity with PI3K-beta based on a 100% homology from human sources with the immunizing sequence.  Expect partial cross reactivity to PIK3C beta from mouse and rat (93% homology) sources.  Cross-reactivity with PIK3C beta from other sources has not been determined.

Immunogen:

Immunogen: This affinity purified PIK3C-ß antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-terminal region of human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-PIK3C beta Antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 122.7 kDa in size corresponding to PI3K beta by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1.0 μg/mL

ELISA Dilution: 1:50,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.09 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details for PIK3CB

Target: PIK3CB (Phosphoinositide-3-Kinase, Catalytic, beta Polypeptide (PIK3CB))

Alternative Name: PIK3CB (PIK3CB Products)

Synonyms: P110BETA antibody, PI3K antibody, PI3KBETA antibody, PIK3C1 antibody, fb92a07 antibody, wu:fb92a07 antibody, 1110001J02Rik antibody, AI447572 antibody, p110beta antibody, PI3Kbeta antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta antibody, phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta antibody, PIK3CB antibody, pik3cb antibody, Pik3cb antibody

Gene ID: 5291

NCBI Accession: NP_006210

UniProt: P42338