EZH1 antibody (Internal Region)

Catalog No. ABIN5706846

Product Details anti-EZH1 Antibody

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EZH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Characteristics:

Synonyms: rabbit anti-EZH1 antibody, KIAA0388, Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1, EZH-1, EZH 1

Background: Anti-Ezh1 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Anti-EZH1 Antibody detects human EZH1. Polycomb group (PcG) proteins are essential epigenetic regulators in normal tissue homeostasis and are involved in transcriptional repression. The PcG members Ezh2 and Ezh1 are important determinants of embryonic stem cell identity, and the transcript levels of these histone methyltransferases are inversely correlated during development. Recent studies have shown that EZH1 also has histone H3K27 methyltransferase activity and binds to an overlapping subset of genes. EZH1 and EZH2 have different expression patterns. EZH2 is found in actively proliferating cells, whereas EZH1 expression is higher in nonproliferative adult tissues. EZH1 partially compensates for the loss of EZH2, as shown in cells lacking only Ezh2. In mice, EZH1 is a regulator of homeotic gene expression implicated in the assembly of repressive protein complexes in chromatin. Anti-EZH1 Antibody is ideal for investigators studying pathogenesis of hematological malignancies.

Gene Name: EZH1

Purification: Anti-EZH1 is directed against human EZH1 at an internal position. This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.  A BLAST analysis was used to suggest reactivity with this protein in mouse, bovine, and orangutan species based on 100% homology for the immunogen sequence.

Immunogen:

Immunogen: Affinity purified Anti-EZH1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human EZH1 protein.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Affinity purified Anti-EZH1 antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band ~86 kDa in size corresponding to Ezh1 by western blotting in the appropriate cell lysate or extract. Testing using positive control NIH/3T3 nuclear extract p/n W10-001-A74.

Western Blot Dilution: 1.0 μg/mL

ELISA Dilution: 1:50,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

Target Details for EZH1

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Alternative Name: Ezh1 (EZH1 Products)

Synonyms: KMT6B antibody, si:ch211-250i3.1 antibody, zgc:136829 antibody, AW557915 antibody, mKIAA0388 antibody, EZH1 antibody, enhancer of zeste 1 polycomb repressive complex 2 subunit antibody, EZH1 antibody, ezh1 antibody, Ezh1 antibody

Gene ID: 2145

NCBI Accession: NP_001982

UniProt: Q92800