Recombinant p21 antibody (full length)

Catalog No. ABIN5707847

This Rabbit Monoclonal antibody specifically detects p21 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Recombinant p21 antibody (full length) (ABIN5707847)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This p21 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: CIP1-2275R

Product Details anti-p21 Antibody

Binding Specificity: full length

Purpose: Recombinant CDNK1A Antibody / p21 (azide and preservative free)

No Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Protein A affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant CDNK1A antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the recombinant CDNK1A antibody to be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant CDNK1A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Alternative Name: p21 / CDKN1A

Background: This mAb recognizes a 21 kDa protein, identified as the p21WAF1 tumor suppressor protein, expressed by the CDNK1A gene. This mAb is highly specific to CDNK1A/p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk's and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of CDNK1A expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).

UniProt: P38936

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy