Recombinant Retinoblastoma Protein (Rb) antibody

Catalog No. ABIN5708007

The Rabbit Monoclonal anti-Retinoblastoma Protein (Rb) antibody is suitable to detect Retinoblastoma Protein (Rb) in samples from Human and Mouse. It has been validated for IHC (p).

Quick Overview for Recombinant Retinoblastoma Protein (Rb) antibody (ABIN5708007)

Target: Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Retinoblastoma Protein (Rb) antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB1-2313R

Product Details anti-Retinoblastoma Protein (Rb) Antibody

Purpose: Recombinant RB1 Antibody / Rabbit Monoclonal

Purification: Protein A affinity chromatography

Immunogen: A human recombinant partial protein was used as immunogen for this recombinant RB1 antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Due to variation in protocol and secondary antibody used, the recombinant RB1 antibody may need to be titered for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: The recombinant RB1 antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Target Details for Retinoblastoma Protein (Rb)

Target: Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Alternative Name: Retinoblastoma / Rb

Background: Recognizes a 105 kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

UniProt: P06400

Pathways: Mitotic G1-G1/S Phases